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目的研究2.2.15细胞中是否存在HBVcccDNA,探讨2.2.15细胞内HBV产物的动态表达规律。方法采用PCR方法检测2.2.15细胞内cccDNA,Taqman定量PCR技术检测2.2.15细胞内及培养上清中HBVDNA含量,EIA方法检测培养上清中HBsAg、HBeAg的动态表达,并进行定量资料相关性统计学分析。结果2.2.15细胞及培养上清中存在cccDNA,2.2.15细胞培养上清中HBVDNA与HBsAg、HBeAg之间存在相关性(r=0.833,P<0.05和r=0.939,P<0.01),而细胞内HBVDNA与培养上清HBVDNA及HBsAg、HBeAg之间无相关性(r=0.024,P>0.05和r=0.177,P>0.05)。结论为阐明2.2.15细胞内HBV的复制规律提供一定依据。
Objective To investigate the presence or absence of HBVcccDNA in 2.2.15 cells and to investigate the dynamic expression of HBV in 2.2.15 cells. Methods The intracellular cccDNA in 2.2.15 cells was detected by PCR and the content of HBVDNA in 2.2.15 cells and culture supernatant was detected by Taqman quantitative PCR. The dynamic expression of HBsAg and HBeAg in culture supernatant was detected by EIA and the correlation of quantitative data Statistical analysis. Results 2.2.15 There was a correlation between HBVDNA and HBsAg and HBeAg in 2.2.15 cell culture supernatant (r = 0.833, P <0.05 and r = 0.939, P <0.01) Intracellular HBVDNA and culture supernatant HBVDNA and HBsAg, HBeAg no correlation (r = 0.024, P> 0.05 and r = 0.177, P> 0.05). Conclusion To clarify the 2.2.15 intracellular HBV replication provides a basis for the law.