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目的:观察地塞米松对哮喘豚鼠体外不同密度嗜酸性粒细胞(Eos)凋亡及Fas mRNA表达的影响,探讨其促进哮喘Eos凋亡的机制。方法:卵蛋白激发哮喘豚鼠动物模型48 h后行支气管肺泡灌洗,分离不同密度Eos。地塞米松(DM)(10-10~10-5mol/L)干预24 h,原位杂交检测Eos的Fas mRNA表达,3’末端脱氧核苷转移酶介导的脱氧三磷酸尿苷(d-UTP)缺口末端标记(TUNEL)法检测细胞凋亡。结果:DM干预24 h,可见Eos凋亡增加,以低密度Eos(HEos)为明显,与对照组相比,在10-9mol/L时出现显著差别(P<0.05);DM可使Eos表达Fas mRNA增加,并具有剂量依赖性;HEos与NEos相比,其Fas mR-NA表达无明显差别;Eos Fas mRNA表达与其细胞凋亡呈正相关。结论:DM可提高Fas mRNA表达,促进Eos凋亡。Fas可能是DM调节Eos凋亡的通路之一。
OBJECTIVE: To observe the effect of dexamethasone on the apoptosis of Eos and the expression of Fas mRNA in asthmatic guinea pigs in vitro, and to explore its mechanism of promoting apoptosis of Eos in asthmatic mice. Methods: The ovalbumin-induced asthmatic guinea pigs were treated with bronchoalveolar lavage for 48 h, and Eos were isolated at different densities. Dexamethasone (DM) (10-10 ~ 10-5mol / L) intervention 24 h, Eos Fas mRNA expression was detected by in situ hybridization, 3’-terminal deoxynucleotidyl transferase mediated deoxyuridine triphosphate (d- UTP) nick end labeling (TUNEL) method to detect apoptosis. Results: The apoptosis of Eos increased with the intervention of DM at 24 h, which was marked by the low density Eos (HEos) compared with the control group at 10-9 mol / L (P <0.05). DM induced the expression of Eos Fas mRNA increased in a dose-dependent manner. Compared with NEos, HE mRNA showed no significant difference in Fas mR-NA expression. Eos Fas mRNA expression was positively correlated with apoptosis. Conclusion: DM can increase the expression of Fas mRNA and promote the apoptosis of Eos. Fas may be one of the pathways by which DM regulates Eos apoptosis.