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利用酸性聚丙烯酰胺凝胶电泳（Ａ－ＰＡＧＥ）方法，鉴定分析了我国部分重要小麦品种或种质醇溶蛋白Ｇｌｉ－１和Ｇｌｉ－２编码位点等位基因组成特点。 ３６个品种的研究结果表明，我国小麦品种在醇溶蛋白几个主要位点上均存在较大的变异度，Ｎｅｉ氏遗传变异系数（Ｈ）达０．７７５。６个位点一共检测到５９个不同的醇溶蛋白等位基因，其中出现频率较高的有８个等位基因，即Ｇｌｉ－Ｂ２ｇ（５５．５６％）、Ｇｌｉ－Ｄｌｋ（５０％）、Ｃｌｉ－Ａｌａ（３３．３３％）、Ｇｌｉ－Ａ２ｆ（３０．５６％）、Ｇｌｉ－Ｂｉｂ（２２．２２％）、ＧｌｉＤｌｆ（２２．２２％）、Ｇｌｉ－Ｂ２ｂ（２２．２２％）和Ｇｌｉ－Ｄ２ｇ（２２．２２％）。国外品种中很少的４个等位基因（Ｇｌｉ－Ｄ１ｂ、Ｇｌｉ－Ａ２ｃ、Ｇｌｉ－Ｂ２ｇ和Ｇｌｉ－Ｄ２ｇ）在我国具有较高的频率。可作为１ＢＬ／１ＲＳ易位标记的Ｇｌｉ－Ｂ１ｌ等位基因在中国品种中的频率较高。另外，还发现一些优质醇溶蛋白等位基因（如Ｇｌｉ－Ｂ１ｂ、Ｇｌｉ－Ｂ２ｃ、Ｇｌｉ－Ａ２ｂ等）在我国品种中出现的频率很低，这可能是中国小麦品种品质普遍较差的一个原因。
The characteristics of the alleles of Gli-1 and Gli-2 encoding loci in some important wheat cultivars or germplasms in China were identified and analyzed by using acidic polyacrylamide gel electrophoresis (A-PAGE). The results of 36 cultivars showed that there was a large variation in several major gliadin loci in Chinese wheat cultivars. The genetic variation coefficient (H) of Nei’s was 0.775.6 and a total of 59 There were 8 alleles with high frequency of occurrence, including Gli-B2g (55.56%), Gli-Dlk (50%) and Cli-Ala (33.33% ), Gli-A2f (30.56%), Gli-Bib (22.22%), GliDlf (22.22%), Gli-B2b (22.22%) and Gli-D2g (22.22%). Few foreign alleles (Gli-D1b, Gli-A2c, Gli-B2g and Gli-D2g) have a high frequency in our country. The Gli-B1l allele, which can be used as a 1BL / 1RS translocation marker, has a higher frequency in Chinese breeds. In addition, it was also found that the occurrence frequency of some gliadin alleles (such as Gli-B1b, Gli-B2c, Gli-A2b, etc.) in Chinese varieties is very low, which may be one of the reasons for the generally poor quality of Chinese wheat varieties .