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In the present study,appropriate nitrogen (N) application mode in Jianghan Plain was explored by investigating the effects of different N applications on the photosynthetic characteristics of flag leaves and nitrogen use efficiency (NUE) in a wheat cultivar Zhengmai 9023.Nitrogen was top-dressed before sowing,before winter,and during the jointing stage,at different ratios:1∶1∶0 (N1),1∶0∶1 (N2),2∶1∶1 (N3),1∶1∶1 (N4),and 0∶0∶1 (N5),under the same amount of total N (180 kg/hm2) during the growing season.No nitrogen fertilizer was used in the control (NO).Results showed that the SPAD values and photosynthetic rate (Pn) of different treatments in flag leaves increased initially and then decreased around the anthesis stage.The two indices in N1 and N5 treatments decreased rapidly after flowering,whereas those in N2,N3,and N4 treatments maintained at high levels for a long period after anthesis.Thus,reasonable nitrogen application could retard the decline of SPAD and Pn after anthesis.N4 and N1 treatments showed large dry matter accumulation.In decreasing order of crop yield,the treatments were:N4＞N1 ＞ N3＞N5＞N2＞N0.The effective panicle number and grain number per spike of N2 were significantly lower than those of other treatments,and there was no significant difference among other treatments.No significant correlation was found between nitrogen application and 1 000-grain weight in this experiment.The nitrogen accumulation,agronomic efficiency of nitrogen fertilizer,nitrogen uptake and use efficiency of above-ground parts,nitrogen uptake and use efficiency of grain of N4 treatment were higher than those of other treatments,but the nitrogen harvest index of N4 was at a low level.In summary,N4 treatment is the most suitable nitrogen application mode in wheat after rice.