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根据已发表的日本小麦黄花叶病毒（ｗｈｅａｔｙｅｌｏｗｍｏｓａｉｃｂｙｍｏｖｉｒｕｓ，ＷＹＭＶ）ＲＮＡ１核苷酸序列，设计了一对引物ＮＩｂ０１／ＮＩｂ０２，用来扩增浙江浦江土传线状小麦黄花叶病毒分离物ＮＩｂ基因的全长序列。以表现典型病症的小麦植株病叶总ＲＮＡ为模板，Ｏｌｉｇｏｄ（Ｔ）１８为引物合成第一链ｃＤＮＡ，用ＲＴＰＣＲ法扩增浦江分离物ＮＩｂ全长基因，并将此基因直接克隆到ｐＧＥＭＴ质粒中，进行全序列测定。结果表明该分离物与日本ＷＹＭＶ相应序列具有很高的同源性，而与小麦梭条斑花叶病毒（ＷＳＳＭＶ）存在很大的差异，因此认为浦江病毒分离物为ＷＹＭＶ。
A pair of primers NIb01 / NIb02 was designed based on the published nucleotide sequence of wheat Yelv mosaic virus (WYMV) RNA1 to amplify the full length of the NIb gene sequence. The total RNA of diseased leaves of typical wheat plants was used as a template. Oligod (T) 18 was used as primer to synthesize the first strand cDNA. The full length NIb gene of Pujiang isolate was amplified by RTPCR and cloned directly into pGEM T plasmid, the whole sequence was determined. The results showed that this isolate had high homology with the corresponding sequence of WYMV in Japan and was quite different from WSSMV. Therefore, the isolate of Pujiang virus was considered to be WYMV.