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以气升式发酵罐代替传统的动态搅拌装置对驱油微生物DSGT进行了发酵工艺条件和发酵参数的研究,通过单因素试验和正交试验得出,当循环泵流量90m3/h、喷雾喷头数量65个、发酵温度65℃、培养基量2%、发酵时间60h、营养因子配比3时,菌浓可达到4.8×108个/ml,且气升式反应器的发酵效果明显好于传统搅拌式反应釜。高效生物活性剂能提高菌种繁殖速度和数量,添加量0.5‰时菌浓可提高到7.8×108个/ml。现场试验表明,注入微生物菌液前后,采出井日产液由41t上升到54.6t,增幅33.1%;日产油由8.9t上升到19.1t,增幅114%;含水由78.2%降到65%;平均每天单井增油0.7t,投入产出比为1:1.99。
The fermentation process conditions and fermentation parameters of DSGT were studied by using air-lift fermenter instead of the traditional dynamic agitator. Through the single-factor test and orthogonal test, when the circulating pump flow rate was 90m3 / h, the number of spray nozzles 65, the fermentation temperature of 65 ℃, the amount of medium 2%, the fermentation time 60h, the ratio of nutrition factor 3, the bacteria concentration can reach 4.8 × 108 / ml, and the airlift reactor fermentation was significantly better than the traditional Type reactor. Efficient bioactive agent can increase the speed and quantity of strain reproduction, bacteria concentration can be increased to 7.8 × 108 个 / ml when added 0.5 ‰. Field tests showed that the daily production of crude oil increased from 41t to 54.6t before and after the injection of microbial broth, increasing by 33.1%; the daily output rose from 8.9t to 19.1t, an increase of 114%; the water content decreased from 78.2% to 65% Single well oil increase 0.7t, input-output ratio of 1: 1.99.