【摘 要】
：
Shedaoenase, a serine protease, was isolated from the venom of Agkistrodon shedaoenthesis Zhao with an apparent molecular mass of 36 kDa. It was purified by aff
【机 构】
：
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Aca
论文部分内容阅读
Shedaoenase, a serine protease, was isolated from the venom of Agkistrodon shedaoenthesis Zhao with an apparent molecular mass of 36 kDa. It was purified by affinity chromatography on arginine Sepharose 4B column and anion exchange on Mono Q fast protein liquid chromatography. Shedaoenase preferentially cleaved the Aα-chain of human fibrinogen and slowly digested the Bβ-chain. It also showed arginyl esterase activity using Nα-benzoyl-L-arginine ethyl ester as a substrate, and some synthetic chromogentic substrates, such as Chromozym PL, S-2266, and S-2160, could also be hydrolyzed. The enzyme activity of shedaoenase could be completely inhibited by phenylmethylsulphonylfluoride and could be little inhibited by the chelating reagent EDTA. The N-terminal sequence of shedaoenase was determined, and its full-length cDNA encoding a protein of 238 amino acid residues was cloned by reverse transcription-polymerase chain reaction from the total mRNA extracted from the snake venom gland. The deduced primary sequence of shedaoenase shares significant homology with other snake venom serine proteases.
其他文献
植物在面对大自然中各种生物胁迫和非生物胁迫时会采用多种机制来适应环境。在植物与自然的相互作用研究过程中,越来越多的学者开始关注植物体在基因水平上的调节作用。近几
(一) 电视广告,为商品经济服务,具有强烈的商品性,这是显而易见的。但是,电视广告作为一种节目,它包容在电视艺术的大框架之内,具有独特的艺术品格,这也是不容忽视的。因此,
Bcl-2/adenovirus E1B 19 kDa interacting protein 2 like-1 (BNIPL-1) is a novel human protein identified in our laboratory, which can interact with Bcl-2 and Cdc4
“水暖水寒鱼自知,花开花落春不管”这句贤文是说鱼儿生活在水里,能够感受到水的冷暧;春天给大地带来了温暖的阳光和蓬勃的生机,至于花儿是开是落,春天是不管的。这句贤文启
Human α-synuclein is a presynaptic terminal protein and can form insoluble fibrils that are believed to play an important role in the pathogenesis of several n
Only β-NGF, the subunit of the 7S NGF complex, exhibits NGF activity, but the function ofthe zinc ion in native β-NGF has received little attention. Flameless
<正> 与世界上其它大部分地区相比,非洲的大众传播媒介并不发达。报纸数量有限,某些国家甚至没有一份日报。几乎没有发行量大一点的日报。除大一点的城镇外,其他地区的读者几乎读不到报。尽管广播电台在每一个非洲国家都有,但他们的转播功率通常很弱,大多数国家的传播网也少。电视自然更不发达,在某些国家,甚至一无所有。至于已经引进电视的地区,主要也是局限于那些有交流电可供运用的城镇中的少数观众。大部分电子大众传播媒介均归政府所控制。1982年,整个非洲大陆上只有三家私人商业
Plant ribosome-inactivating proteins (RIPs) have multiple biological functions, and have beenwidely used in the studies on biomedical and agronomic applications
茄子是一种重要的蔬菜作物,利用小孢子培养技术进行育种材料创制具有重要意义。但是,茄子小孢子离体培养脱分化及胚状体产生过程很不稳定,易出现发育停滞、诱导率低等现象,因
本试验采用水培方式对氮、磷、钾及不同氮素形态配比对薄荷精油及其品质的影响进行了研究。本研究对氮、磷、钾和不同氮素形态及其配分别设置五个浓度梯度,研究不同浓度氮、