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微RNAs(又称miRNAs或miRs)是一类长度为19~24个核苷酸的单链非编码RNA分子.miRNA通过与其靶向的mRNA分子序列特异性互补配对,调节mRNA表达水平,抑制转录后的蛋白质翻译.miRNA在肿瘤中既可作为致癌因子也可作为抑癌因子.本研究前期已报道miR-26b在前列腺癌细胞系中低表达,并且抑制细胞自噬.本研究进一步全面揭示miR-26b对前列腺肿瘤细胞的作用.我们发现过表达miR-26b能够在体外抑制前列腺癌细胞的增殖和侵袭,并抑制裸鼠体内原位异种前列腺肿瘤的生长.为了探究miR-26b对前列腺癌细胞增殖和侵袭的潜在调控机制,我们进行了表达谱芯片鉴定miR-26b调控基因.表达谱芯片分析表明,在前列腺癌细胞系PC-3中过表达miR-26b后,显著上调的基因57个,显著下调的基因55个(变化倍数均大于2,且P值小于0.05).差异基因的功能多与细胞增殖、凋亡调控、蛋白质磷酸化和泛素化修饰调控过程相关,并且富集在多种信号通路中,例如TNF和TGF-β信号通路.在这些筛选出的基因中,CEACAM6表达水平下调2.17倍;序列分析及实验验证表明,CEACAM6的3′UTR区存在miR-26b的互补序列,是miR-26b的直接靶标.本研究证明了miR-26b能够靶向结合抑制CEACAM6的表达,从而抑制前列腺癌细胞在体外和体内的细胞增殖和侵袭活性,miR-26b是前列腺癌中的抑癌microRNA.
MicroRNAs (also known as miRNAs or miRs) are a class of single-stranded, non-coding RNA molecules 19 to 24 nucleotides in length that specifically regulate mRNA expression and inhibit transcription by specifically pairing with targeted mRNA sequences After the protein translation.miRNA in cancer as both oncogenic factor can also be used as a tumor suppressor factor in this study has been reported in the early miR-26b prostate cancer cell lines in the low expression and inhibition of cell autophagy.This study further fully reveal the miR -26b on prostate tumor cells.We found that overexpression of miR-26b can inhibit the proliferation and invasion of prostate cancer cells in vitro and inhibit the growth of in situ xenograft tumors in nude mice.In order to explore the effect of miR-26b on prostate cancer cells Proliferation and invasion, we performed the expression profiling to identify the miR-26b regulatory genes.Expression profiling revealed that 57 genes were significantly upregulated after miR-26b was overexpressed in the prostate cancer cell line PC-3, 55 genes were significantly downregulated (the multiple of which was greater than 2, and the P value was less than 0.05) .Differences in the gene function and cell proliferation, apoptosis regulation, protein phosphorylation and ubiquitination modification regulation process And enriched in a variety of signaling pathways, such as TNF and TGF-β signaling pathway in these selected genes, CEACAM6 expression decreased 2.17 times; sequence analysis and experimental verification showed that CEACAM6 3’UTR region exists The complement of miR-26b is the direct target of miR-26b.This study demonstrates that miR-26b can target binding to inhibit the expression of CEACAM6, thereby inhibiting the proliferation and invasion activity of prostate cancer cells in vitro and in vivo. miR-26b Is a tumor suppressor microRNA in prostate cancer.