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目的探索卵巢移植中的移植物（胎儿卵巢）的体外保存方法。方法将１６例２０～２８周的胎儿卵巢置于含１０％二甲基亚砜（ＤＭＳＯ）的培养液中，采用三步冷冻法投入液氮保存。复温时采用４０℃水浴快速复温和室温下慢复温，然后进行组织培养观察卵泡发育和雌二醇（Ｅ２）分泌情况。结果胎儿卵巢复温组培养，其卵泡能进一步发育并产生Ｅ２；快复温组卵泡组织形态良好，并能维持良好的内分泌功能；慢复温组卵巢上皮破裂，Ｅ２分泌量明显减少。结论胎儿卵巢冷冻保存后，快速复温能更好地维持卵泡形态发育和内分泌功能。
Objective To explore the in vitro preservation of the graft (fetal ovary) in ovarian transplantation. Methods Twenty-six fetuses ovariectomized from 20 to 28 weeks were placed in culture medium containing 10% dimethylsulfoxide (DMSO) and stored in liquid nitrogen by three-step freezing. During rewarming, the cells were rapidly rewarmed in a water bath at 40 ° C and slowly rewarmed at room temperature. Then the tissue culture was used to observe the follicular development and estradiol (E2) secretion. Results Fetal ovary rewarming group cultured, the follicles can further develop and produce E2; fast rewarming group follicular tissue morphology and maintain good endocrine function; slow rewarming group ovarian epithelial rupture, E2 secretion was significantly reduced. Conclusion After cryopreservation of fetal ovary, rapid rewarming can better maintain the development of follicular morphology and endocrine function.