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目的研究细梗香草皂苷B(capilliposide B,LC-B)和细梗香草皂苷C(capilliposide C,LC-C)在不同温度,pH条件下水解动力学,及水解产物的鉴定。方法采用高效液相色谱法测定LC-B,LC-C含量,推导出LC-B,LC-C水解过程,恒温30,50和70℃,pH分别为9.0,10.0,11.0的条件下,不同时间点分别取样检测LC-C和LC-B的含量,探讨其稳定性,并通过质谱、核磁共振谱鉴定水解产物。结果 LC-B,LC-C在不同温度,pH条件下的水解反应均符合一级动力学。水解速率受水解的温度和体系pH值的影响:相同的水解温度,pH越高,水解越快;相同pH,温度越高水解越迅速。水解产物为细梗香草皂苷A(capilliposide A,LC-A)。结论该实验为LC-A的制备提供一种有效方法。
Objective To study the hydrolysis kinetics of capilliposide B (LC-B) and capilliposide C (LC-C) under different temperature and pH conditions and the identification of hydrolyzate. Methods The contents of LC-B and LC-C were determined by high performance liquid chromatography (LC-C), and the LC-B and LC-C hydrolysis processes were deduced. Under the conditions of constant temperature of 30, 50 and 70 ℃, pH of 9.0, At the time point, the content of LC-C and LC-B were sampled and analyzed respectively, and their stability was discussed. The hydrolyzate was identified by mass spectrum and nuclear magnetic resonance spectrum. Results The hydrolysis of LC-B and LC-C at different temperatures and pHs all accord with the first-order kinetics. The rate of hydrolysis is affected by the temperature of hydrolysis and the pH of the system: the same hydrolysis temperature, the higher the pH, the faster the hydrolysis; the same pH, the higher the temperature, the faster the hydrolysis. The hydrolyzate was capilliposide A (LC-A). Conclusion This experiment provides an effective method for the preparation of LC-A.