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利用多巴胺对纳米TiO_2进行表面修饰,制备了仿生粘附纳米TiO_2用于脂肪酶的固定化,固载率达242.38mg/g。采用扫描电子显微镜(SEM)、傅里叶变换红外光谱仪(FT-IR)、X射线衍射仪(XRD)等手段对固载酶界面进行表征:SEM和XRD表征表明纳米TiO_2固载酶后粒径增大20nm左右,且吸附前后纳米TiO_2的晶型变化不大;FT-IR表征中固载酶在1647cm-1处出现了CN键特征峰,1629cm-1出现了芳香族CC键特征峰,同时热分析中在200~500℃温度区间多巴胺和脂肪酶发生了热分解。通过仿生纳米TiO_2表面醌类物质与酶分子中氨基共价结合实现了对酶的固载。
The surface modification of nano-TiO 2 with dopamine was carried out to prepare biomimetic nano-TiO 2 for immobilization of lipase with the immobilization rate of 242.38 mg / g. The immobilized enzyme interface was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and X-ray diffractometer (XRD). The SEM and XRD results showed that the particle size And the crystal form of nano-TiO2 did not change much before and after adsorption. FT-IR characterization showed that the CN bond peak appeared at 1647cm-1 and the aromatic CC bond appeared at 1629cm-1 At the same time, thermal decomposition of dopamine and lipase occurred in the temperature range of 200 ~ 500 ℃. Immobilization of the enzyme was achieved by the covalent bonding of quinone with biomolecule amino groups on the surface of biomimetic TiO 2.