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目的探讨甲状旁腺素(parathyroid hormone,PTH)基因多态性与广东地区部分汉族女性人群骨密度(bone mineral density,BMD)的相关性。方法选取广东成年女性617例,年龄30～75岁,平均(47.82±13.57)岁,用聚合酶链反应及限制性片段长度多态性技术检测外周血白细胞基因组护骨素基因型,采用双能X线吸收测定其腰椎侧位、股骨颈、粗隆间、大转子、ward s三角等部位的骨密度值。结果617例受试对象中,PTH基因BB型447例(72.4%);Bb型157例(25.4%);和bb型13例(2.1%)。绝经前妇女PTH基因多态性频率与绝经后妇女比较差异无统计学意义。无论是整个受试群体,还是将其分为绝经前及绝经后群体,其基因及基因型分布均符合Hardy-Wenbeng平衡定律。经协方差分析校正了年龄、体质指数等因素后(对绝经后妇女人群,同时对绝经年限进行校正)发现,3种不同的基因型相互之间的BMD变化均差异无统计学意义(P>0.05)。结论PTH基因多态性与广东地区汉族妇女人群BMD关系不密切,不能作为筛查和预示骨质疏松症的遗传易感位点。
Objective To investigate the relationship between parathyroid hormone (PTH) gene polymorphisms and bone mineral density (BMD) in some Han female population in Guangdong. Methods A total of 617 adult female patients aged 30-75 years (mean age 47.82 ± 13.57 years) were selected. The genotypes of osteoprotegerin in peripheral blood leucocytes were detected by polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) X-ray absorptiometry measured the lumbar lateral mass, femoral neck, intertrochanteric, greater trochanter, ward s triangle and other parts of the bone mineral density value. Results Among the 617 subjects, 447 (72.4%) were PTH genotype BB, 157 (25.4%) were Bb, and 13 (2.1%) were bb. The frequency of PTH gene polymorphism in premenopausal women was not significantly different from that in postmenopausal women. Whether it is the entire test group, or divided into pre-menopausal and postmenopausal groups, the gene and genotype distribution are in line with the Hardy-Wenbeng equilibrium law. Covariance analysis adjusted for age, body mass index and other factors (postmenopausal women population, while menopause correction) found that there was no significant difference in BMD changes between the three genotypes (P> 0.05). Conclusion The genetic polymorphism of PTH is not closely related to the BMD of Han women in Guangdong Province. It can not be used as a genetic predisposition to screen and predict osteoporosis.