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目的:观察参麦汤对糖耐量低减(Impaired Glucoseolerance,IGT)大鼠脂联素(Adiponectim)、瘦素(leptin)及脂联素受体R2mRNA表达的影响。方法:高脂高糖饲料喂养结合腹腔注射小剂量链脲佐菌素溶液的方法复制糖耐量低减大鼠模型;用参麦汤治疗;实验结束后颈动脉取血,采用酶免法测血清Adiponectim及leptin,应用RT-PCR方法测定大鼠肝组织脂联素受体R2mRNA的表达。结果:和正常组比较,模型组Adiponectim浓度降低,有显著性差异(P<0.05),leptin的浓度升高,有极显著性差异(P<0.001);与模型组比较,各干预组均可提高Adiponectim浓度、降低leptin水平,其中以参麦汤加运动组为著(P<0.01),参麦汤组(P<0.05)、运动组(P<0.05)亦有明显差异;与正常组相比,模型组大鼠脂联素受体R2mRNA表达明显降低(P<0.001),各治疗组与模型组相比,参麦汤加运动组、参麦汤组大鼠脂联素受体R2mRNA表达上升显著(P<0.001),运动组(P<0.05)次之。结论:在IGT阶段已经出现leptin、Adiponectim分泌异常,参麦汤能显著地降低leptin水平,提高Adiponectim水平,促进脂代谢,上调糖耐量异常大鼠肝脂联素受体R2mRNA表达。推测参麦汤能改善机体能量的调节和维持代谢稳态。
Objective: To observe the effects of Shenmaitang on the expression of Adiponectim, leptin and adiponectin receptor R2mRNA in rats with impaired glucose tolerance (IGT). Methods: The model of impaired glucose tolerance was induced by high fat and high glucose diet combined with intraperitoneal injection of low dose streptozotocin solution. The rats were treated with Shen Mai Tang. The carotid arteries were harvested after the experiment, Adiponectim and leptin, the expression of adiponectin receptor R2 mRNA in rat liver tissue was detected by RT-PCR. Results: Compared with the normal group, the concentration of Adiponectim in the model group decreased significantly (P <0.05) and the concentration of leptin increased significantly (P <0.001). Compared with the model group, all the intervention groups (P <0.01), Shenmai Decoction group (P <0.05) and exercise group (P <0.05). Compared with the normal group, the Adiponectim concentration and the leptin level were significantly decreased , R2 mRNA expression of adiponectin receptor in model group was significantly lower than that in model group (P <0.001). Compared with model group, the expression of R2 mRNA in adiponectin receptor increased significantly in Shenmang Tonga exercise group and Shenmai Tang group (P <0.001), and exercise group (P <0.05). CONCLUSION: Leptin and Adiponectim secretion abnormalities have occurred in IGT stage. Shenmai Tang can significantly decrease leptin level, increase Adiponectim level, promote lipid metabolism and upregulate the expression of R2 mRNA in hepatic adiponectin receptor in rats with impaired glucose tolerance. Presumably Shenmai Tang can improve the body’s energy regulation and maintain metabolic homeostasis.