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目的了解2,3,7,8-四氯二苯并二噁英(TCDD)对雄性大鼠生殖系统的影响。方法清洁级Wistar雄性大鼠32只,体重(100±10)g,随机分成4组,每组8只,连续经口灌胃染毒90 d,剂量分别为2.5,25,250 ng/kg,对照组给予二甲基亚砜(DMSO),测定血清中睾酮(T)、促黄体生成素(LH)、促卵泡生成素(FSH)的激素水平以及睾丸、前列腺、精囊腺的脏器质量系数,附睾尾精子畸形率。结果与对照组比较,各染毒组T水平下降,差异有显著性(P<0.05);各染毒组FSH和LH水平上升,但差异无显著性(P>0.05);中、高剂量组的睾丸、精囊腺及所有染毒组的前列腺脏器质量系数均低于对照组(P<0.05);各染毒组精子畸形率随剂量的增加而上升,中、高剂量组与对照组相比差异显著(P<0.01)。结论TCDD亚慢性染毒,可影响精子和生殖系统的正常发育,并在一定程度上对生殖激素的稳态造成了干扰。
Objective To investigate the effects of 2,3,7,8-tetrachlorodibenzodioxin (TCDD) on the reproductive system in male rats. Methods Totally 32 Wistar male rats were randomly divided into 4 groups (n = 8), with a body weight of (100 ± 10) g. The rats were orally administrated orally for 90 days at dosages of 2.5, 25 and 250 ng / kg, The levels of testosterone (T), luteinizing hormone (LH) and follicle-stimulating hormone (FSH) in serum were measured and the organ coefficient of testis, prostate and seminal vesicle were measured. Tail sperm deformity rate. Results Compared with the control group, the levels of T decreased, the difference was significant (P <0.05). The levels of FSH and LH increased in all treatment groups, but the difference was not significant (P> 0.05) (P <0.05). The sperm deformity rate increased with the increase of dose in the testis, seminal vesicle and all the exposed groups. The medium and high dose groups and the control group The difference was significant (P <0.01). Conclusion Subchronic exposure to TCDD can affect the normal development of sperm and reproductive system, and to a certain extent, interfere with the steady state of reproductive hormone.