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为了深入研究棉花JAZ基因在低温响应中的作用机理,利用RT-PCR方法从陆地棉中棉所36中克隆出一个低温应答基因GhJAZ1(GenBank登录号KJ562212).全长CDS序列为810 bp,编码270个氨基酸,预测编码蛋白质的分子量为29.808 ku,理论等电点为8.33,为非跨膜蛋白.该基因编码蛋白具有一个N端NT结构域、一个TIFY结构域和一个C端Jas结构域.系统进化树分析发现,该蛋白与可可树和黄麻的亲缘关系最近.荧光实时定量PCR分析发现,GhJAZ1基因在下胚轴和根中显著高表达.此外,该基因还受到脱落酸(ABA)和茉莉酸甲酯(MeJA)的诱导表达,表达量变化趋势均为先升高后降低,但在MeJA诱导下该基因的表达量变化更明显.在低温胁迫下,不同低温耐性棉花材料中该基因的表达量变化程度也不同,耐低温品种中棉所36中GhJAZ1基因的上调表达更为显著,推测该基因参与了棉花低温胁迫防御反应.亚细胞定位显示,GhJAZ1蛋白定位于细胞核中.该研究可为进一步开展GhJAZ1基因低温响应的分子机制研究奠定基础.“,”To further study the mechanism of cotton JAZ genes in low temperature response,a cold response gene GhJAZ1 was cloned from the upland cotton Zhongmiansuo 36 by RT-PCR (GenBank accession number KJ562212).The CDS sequence of GhJAZ1 was 810 bp in length and encoded 270 amino acids.The predicted mo-lecular weight of this protein was 29.808 ku,and the theoretical isoelectric point was 8.33.It was not a transmem-brane protein.The encoded protein contained a N-terminal NT domain,a TIFY domain,and a C-terminal Jas do-main.Phylogenetic tree analysis showed that GhJAZ1 was much closer to that in Theobroma cacao and Corchorus oli-torius.Quantitative real-time PCR analysis showed that GhJAZ1 had significantly high expression in hypocotyls and roots.In addition,the expression of GhJAZ1 was also induced by abscisic acid(ABA) and methyl jasmonate (Me-JA).The trend of expression was first raised and then decreased. Under MeJA treatment,the gene expression var-ied more obviously. The expression pattern of GhJAZ1 varied in different cold resistant cotton materials under cold stress. The up-regulated expression of GhJAZ1 gene in cold-resistant cotton variety-Zhongmiansuo 36 was more sig-nificant,which suggesting that GhJAZ1 gene may be involved in cold response in cotton. Subcellular localization showed that GhJAZ1 protein was localized in the nucleus.This study lay foundation for further researches on the mo-lecular mechanism of GhJAZ1 in cold response.