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对诱导１ｄ、３ｄ、５ｄＬＡＫ细胞作ＡＮＡＥ、ＡＣＰ、ＰＯＸ及ＰＡＳ细胞化学染色，同时采用ＬＤＨ释放法对ＬＡＫ细胞的细胞毒性进行了检测。结果显示，随诱导天数的延长，ＬＡＫ细胞胞质中ＡＮＡＥ、ＡＣＰ和ＰＡＳ的反应物逐渐增多、增强，阳性率也逐渐增高，不同天数组间有显著性差异（Ｐ＜０．００１）。ＰＯＸ均为阴性反应。细胞毒性试验证实，随诱导天数的延长ＬＡＫ细胞杀伤活性逐渐增强。这表明ＬＡＫ细胞的细胞化学活性与其细胞毒性密切相关，为了解ＬＡＫ细胞的激活状态提供了形态学依据。
The cytotoxicity of LAK cells was detected by chemiluminescence staining of ANAE, ACP, POX and PAS on day 1, day 3 and day 5, and LDH release assay. The results showed that the number of reactants of ANAE, ACP and PAS in LAK cell cytoplasm gradually increased with the induction of lengthening, and the positive rate also increased gradually. There was a significant difference between different days (P <0.001). POX are negative reactions. Cytotoxicity test confirmed that with the induction of prolonged LAK cell killing activity gradually increased. This indicates that the cytochemical activity of LAK cells is closely related to its cytotoxicity and provides a morphological basis for understanding the activation state of LAK cells.