High throughput monoclonal antibody generation by immunizing multiple antigens

• 作 者: LIU Ying WANG YunDan LIU Jing
• 机 构: StateKeyLaboratoryofProteomics,BeijingInstitutesofLifeScience,AnhuiMedicalUniversity
• 出 处: 中国科学：生命科学英文版
• 发表日期: 2014年7期
• 关键词: 单克隆抗体 免疫抗原 高通量 酶联免疫吸附测定法 蛋白质组学 固体培养基 细胞融合 免疫小鼠 high throughput mAb multiple anti
• 基金项目: supported by the National Basic Research Program of China （2011CB915502）, State Key Laboratory of Proteomics Project （SKLP Y200907）, National High Technology Research and Development Program of China

Recognizing proteins via the production of highly specific monoclonal antibodies(mAbs)is crucial to identifying proteins for proteomic research.However,traditional mAb generation is time-consuming with low efficiency.In this study,we assessed the high thr