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目的:探讨胰腺癌细胞对5-Fu和吉西它滨的获得性耐药后bcl-xL和mcl-1表达的变化.方法:应用SRB方法检测5-Fu和吉西它滨对胰腺癌细胞株Panc-1,Mia-Paca-2和Capan-1的细胞毒性作用,应用Western blot法来检测bcl-xL和mcl-1的蛋白表达水平.结果:5-Fu和吉西它滨对3株胰腺癌细胞均产生了细胞毒性作用, 5-Fu长期作用后, Capan-1细胞IC50上升了2.1倍(P<0.05).吉西它滨长期作用后,Capan-1细胞IC50上升了1.5倍(P<0.05).5-Fu或吉西它滨长期作用后,产生了获得性耐药的胰腺癌细胞bcl-xL和mcl-1表达水平上调.结论:5-Fu或吉西它滨长期作用后,产生了获得性耐药,这种耐药与bcl-xL和mcl-1的过度表达相关.“,”Objective:To study the relationships between the acquired resistance of Gemcitabine and 5-Fu and the expression of bcl-xL and mcl-1 in pancreatic cancer cell lines.Methods:The cytotoxic effects of 5-Fu and Gemcitabine in Panc-1,Mia-Paca-2 and Capan-1 pancreatic cancer cells were assessed using Sulforhodamine B methods and expression levels of anti-apoptotic genes of the bcl-xL and mcl-1 were analyzed by Western blot.Results:5-Fu and Gemcitabine displayed cytotoxic effects towards three pancreatic cancer cell lines.After recurrent treatment with 5-Fu,the IC50 values in Capan-1 cells increased 2.1 fold compared to their parental cells (P<0.05).Following recurrent treatment with Gemcitabine,the IC50 values in Capan-1 cells increased 1.5 fold (P<0.05).Western blot analysis show that aftter recurrent treatment with 5-Fu and Gemcitabine the expression of bcl-xL and mcl-1 upgraded.Conclusion:Overexpression of bcl-xL and mcl-1 following recurrent exposure to 5-Fu or Gemcitabine are associaed with the acqurired these drugs.