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目的研究两歧双歧杆菌介导的铜绿假单胞菌外膜蛋白Ⅰ(Bb-OprⅠ)疫苗免疫及PA01株铜绿假单胞菌攻击后,小鼠肺细菌负荷和脾细胞的增殖、T细胞亚群分布及细胞凋亡情况。方法将5×10~9个菌落形成单位(CFU)疫苗灌胃接种BALB/c小鼠,每周3次,连续接种3周。在首次免疫后4周,用5×10~6个CFU的PA01株滴鼻攻击。在攻击后2周,处死小鼠取肺和脾脏,计数肺细菌负荷;MTT法检测脾细胞增殖,流式细胞术检测脾CD4~+T细胞亚群和CD8+T细胞亚群及脾细胞凋亡率。结果 Bb-OprⅠ疫苗免疫及PA01株铜绿假单胞菌攻击后,小鼠肺细菌菌落数减少,脾细胞增殖和CD4~+T细胞比例明显增加,细胞凋亡明显减少。结论重组Bb-OprⅠ疫苗灌胃接种可增加CD4~+T细胞比例,增强对铜绿假单胞菌的抑制作用。
Objective To study the effects of Bifidobacterium bifidum-mediated immunity against Pseudomonas aeruginosa outer membrane protein Ⅰ (Bb-OprⅠ) and PA01-induced Pseudomonas aeruginosa (P. aeruginosa) challenge on lung bacterial load and spleen cell proliferation. T cells Subpopulation distribution and apoptosis. Methods BALB / c mice were inoculated intraperitoneally with 5 × 10 ~ 9 colony forming units (CFU) vaccine three times a week for 3 weeks. Four weeks after the first immunization, PA01 strain was challenged intranasally with 5 × 10 ~ 6 CFU. Two weeks after the challenge, the mice were sacrificed and the lungs and spleens were sacrificed. The lung bacterial load was counted. The proliferation of spleen cells was measured by MTT assay. The subsets of splenic CD4 ~ + T cells and CD8 + T cells and splenocytes were detected by flow cytometry Death rate. Results After Bb-OprI vaccination and PA01-induced Pseudomonas aeruginosa challenge, the number of lung bacterial colonies in mice decreased, the proliferation of spleen cells and the proportion of CD4 ~ + T cells were significantly increased, and the apoptosis rate was significantly decreased. Conclusion Recombinant Bb-Opr Ⅰ vaccination can increase the proportion of CD4 ~ + T cells and enhance the inhibitory effect on Pseudomonas aeruginosa.