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目的：确定与细胞内蛋白质酪氨酸磷酸化相关的激酶。方法：用粒－巨噬系集落刺激因子（ＧＭ－ＣＳＦ）和红细胞生成素（Ｅｐｏ）依赖细胞株ＵＴ－７以及Ｅｐｏ依赖细胞株ＵＴ－７／Ｅｐｏ进行酪氨酸磷酸化的研究。结果：当ＧＭ－ＣＳＦ或Ｅｐｏ刺激其生长依赖细胞时可快速诱导分子量为１４５０００，１３００００，８００００和４００００等多种蛋白质酪氨酸磷酸化。鉴定了分子量为１３００００酪氨酸磷酸化的蛋白质为ＪＡＫ２，一种非受体型酪氨酸激酶。同时，ＧＭ－ＣＳＦ或Ｅｐｏ刺激亦可激活ＪＡＫ２激酶活性。酪氨酸磷酸化和激活ＪＡＫ２只发生在ＧＭ－ＣＳＦ或Ｅｐｏ生长依赖细胞。结论：ＪＡＫ２信号传导途径在ＧＭ－ＣＳＦ和Ｅｐｏ诱导的细胞增殖中起重要作用。
Purpose: To identify kinases associated with intracellular protein tyrosine phosphorylation. Methods: The tyrosine phosphorylation of GM-CSF and erythropoietin-dependent cell line UT-7 and Epo-dependent cell line UT-7 / Epo were studied. Results: When GM-CSF or Epo stimulated their growth-dependent cells, they could rapidly induce a variety of protein tyrosine phosphorylation with molecular weights of 145,000, 130,000, 80,000 and 40,000. A protein of tyrosine phosphorylation at 130000 was identified as JAK2, a non-receptor tyrosine kinase. In the meantime, stimulation with GM-CSF or Epo also activates JAK2 kinase activity. Tyrosine phosphorylation and activation JAK2 occurs only in GM-CSF or Epo growth-dependent cells. Conclusion: The JAK2 signaling pathway plays an important role in GM-CSF and Epo-induced cell proliferation.