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目的 从电镜和免疫电镜水平 ,探讨人类肝癌和肝硬化肝组织存在如动物肝癌形成实验中的卵圆细胞的可能性。方法 对 10例人肝细胞肝癌的手术标本及其癌旁肝硬化组织作了电镜超微结构观察 ,并用胆管上皮分化抗体CK7和肝细胞分化抗体白蛋白对以上组织作超薄切片免疫电镜标记。结果 电镜下 ,所有肝癌(10例 )和肝硬化 (10例 )组织均可找到与我们在人肝母细胞瘤、胆道闭锁肝中所见的三型小上皮细胞。这类细胞卵圆形 ,位于肝癌肿瘤边缘和增生的胆小管内 ,在肝硬化组织则位于汇管区及再生的肝结节内。免疫电镜下 ,三型卵圆细胞均表达CK7和白蛋白 ,但Ⅰ型和Ⅱ型表达CK7多些 ,Ⅲ型表达白蛋白多些。结论 人肝细胞肝癌和肝硬化肝组织中存在卵圆细胞 ,其形态和免疫表型特点与动物致癌模型肝中卵圆细胞一致。结果进一步支持肝前体细胞或干细胞样细胞的假设
Objective To investigate the possibility of existence of oval cells in human hepatocellular carcinoma and liver cirrhosis tissues such as animal hepatoma formation from electron microscopy and immunoelectron microscopy. Methods Electron microscopy was performed on 10 specimens of hepatocellular carcinoma of the liver and their pericarcinomatous cirrhotic tissues. Immuno-microscopy was performed on the above tissues with ultrathin sections by using cholangiocyte differentiation antibody CK7 and hepatocyte differentiation albumin. Results Under electron microscopy, all three types of small epithelial cells seen in human hepatoblastoma and biliary atresia were found in all liver cancer (10 cases) and cirrhosis (10 cases). These cells are oval, located in the edge of the liver cancer and hyperplastic bile duct in liver cirrhosis tissue is located in the portal area and the regeneration of the liver nodules. Immunoelectron microscopy, three types of oval cells were expressed CK7 and albumin, but type Ⅰ and type Ⅱ CK7 more expression, type Ⅲ more albumin. Conclusion There are oval cells in human hepatocellular carcinoma and liver cirrhosis. The morphological and immunophenotypic characteristics are consistent with the oval cells in liver of animal carcinogenic model. The results further support the hypothesis that liver precursors or stem cell-like cells