论文部分内容阅读
目的筛选肝硬化和肝细胞癌(HCC)共同表达的差异基因。方法按TRIzol法分别抽提2例肝硬化、2例HCC和1例正常肝组织总RNA,逆转录合成掺入生物素标记cDNA合成探针,应用基因芯片进行肝硬化及HCC的相关基因表达谱差异分析,扫描芯片荧光信号图像,计算机分析,比较三种组织基因表达谱差异。结果发现2例肝硬化组织中共有1424条基因(9.82%)表达差异;2例HCC组织中共有2756条基因(19.01%)表达差异;而2例肝硬化与2例HCC组织中有851条基因(5.87%)共同表达差异,其中共同上调基因有649条,共同下调基因有202条。结论基因芯片技术能快速筛选肝硬化及HCC相关基因,分析这些共同差异性表达的基因,为阐明肝硬化及HCC复杂的生物学特性与基因表达之间的内在联系,寻找识别肿瘤的标记物提供了可能。
Objective To screen differentially expressed genes co-expressed in cirrhosis and hepatocellular carcinoma (HCC). Methods Total RNA was extracted from 2 patients with liver cirrhosis, 2 patients with HCC and 1 patient with normal liver tissue by TRIzol method. The biotin labeled cDNA synthesis probe was reverse transcribed and gene chips were used to analyze the gene expression profiles of cirrhosis and HCC Difference analysis, scanning fluorescence signal chip images, computer analysis, comparison of three kinds of tissue gene expression differences. The results showed that a total of 1424 genes (9.82%) were expressed in 2 cirrhotic cases. There were 2756 genes (19.01%) in 2 cases of HCC, while 2 cases of cirrhosis and 2 cases of HCC had 851 genes (5.87%), which shared 649 common up-regulated genes and 202 common down-regulated genes. Conclusion Gene chip technology can rapidly screen cirrhotic and HCC related genes and analyze these genes with different expression. In order to elucidate the inherent relationship between the complicated biological characteristics of cirrhosis and HCC and gene expression, find the marker for tumor identification It is possible.