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小麦秆锈病是一种专化性很强的大区远距气传病害,曾造成多个小麦种植国家和地区的毁灭性损失,新的强毒力小种Ug99含有对Sr31等多个重要抗秆锈基因的联合毒性,对我国的小麦生产有巨大潜在威胁,因此,加强小麦秆锈菌生理小种的监测和鉴定是有效防治该病害的基础性研究工作和关键环节。现代分子生物学的迅猛发展,为许多研究提供了新的方法和手段,分子标记技术在区分小麦秆锈菌生理小种方面显示了充分的可行性。本研究利用25对SSR引物对7个小麦秆锈菌主要生理小种进行DNA多态性分析,结果显示,所有特异引物对秆锈菌的扩增结果均呈现出丰富的多态性,秆锈菌的不同生理小种之间存在遗传差异。其中引物SSR180在21C3CPH中扩增出205bp的特异性条带;引物SSR6在Ug99中扩增出170bp的特异性条带,经过多次的重复试验,这些特异性条带均能够比较稳定地重复出现,说明引物SSR180和SSR6可用于小种21C3CPH和Ug99的特异性检测。
Wheat stem rust is a highly specialized long-range airborne disease that has caused devastating losses in several wheat growing countries and regions. The new virulence race Ug99 contains multiple important anti-Sr31 The combined toxicity of stalk rust genes has great potential threat to the wheat production in our country. Therefore, the monitoring and identification of the physiological races of wheat stalk rust is the basic research work and key link for the effective control of this disease. The rapid development of modern molecular biology has provided new methods and measures for many studies. The molecular marker technology has shown sufficient feasibility in distinguishing wheat races from P. rust. In this study, 25 pairs of SSR primers were used to analyze the DNA polymorphism of seven major races of wheat stripe rust. The results showed that the amplified results of all the specific primers showed a rich polymorphism, There is a genetic difference between different races of bacteria. Among them, primer SSR180 amplified a 205bp specific band in 21C3CPH. Primer SSR6 amplified a specific band of 170bp in Ug99. After repeated experiments, these specific bands could be stably repeated , Indicating that primers SSR180 and SSR6 can be used for the specific detection of races 21C3CPH and Ug99.