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共轭聚合物纳米粒子(CPNs)因其高荧光亮度、低毒性、表面易修饰的特性,近年来在生物材料和生物医药领域备受关注。本论文中我们设计、合成了一种新的pH值响应共轭聚合物(PFPA),并通过纳米沉淀方法制备了其纳米粒子。动态光散射实验表明PFPA纳米粒子在水中分散性较好,其粒径约为8nm。PFPA纳米粒子的最大吸收峰为379nm,其摩尔吸光系数为2.1×106 L·mol-1·cm-1;另外该纳米粒子的荧光最大发射峰为422nm,其荧光量子产率为35%。PFPA纳米粒子在汞灯(100瓦)照射下表现出较好的光稳定性,另外MTT实验表明其具有较低的细胞毒性。该纳米粒子具有pH响应的光学特性,并可以用于活细胞成像。PFPA纳米粒子在癌症诊断、药物与基因传递等方面具有潜在的应用价值。
Conjugated polymer nanoparticles (CPNs) have attracted much attention in the fields of biomaterials and biomedicine for their high fluorescence brightness, low toxicity and easy surface modification. In this dissertation, we designed and synthesized a new pH-responsive conjugated polymer (PFPA) and prepared its nanoparticle by nanoprecipitation. Dynamic light scattering experiments showed that PFPA nanoparticles dispersed well in water with a particle size of about 8 nm. The maximum absorption peak of PFPA nanoparticles was 379 nm with a molar absorptivity of 2.1 × 106 L · mol-1 · cm-1. The maximum emission peak of PFPA nanoparticles was 422 nm and the fluorescence quantum yield was 35%. PFPA nanoparticles showed good photostability under the irradiation of mercury lamp (100 watts). In addition, the MTT assay showed that the PFPA nanoparticles had lower cytotoxicity. The nanoparticles have the pH-responsive optical properties and can be used for living cell imaging. PFPA nanoparticles have potential applications in cancer diagnosis, drug delivery and gene delivery.