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目的:观察益肝降脂方药对酒精性脂肪肝病(AFLD)大鼠的早期干预效应。方法:85只雄性SD大鼠随机分为模型组25只,中药组、西药组各30只,连续12周予高脂饲料联合梯度白酒灌胃建立AFLD模型,自第9周依次分别灌服2ml的生理盐水、益肝降脂方药和易善复混悬液;于第4、8周末分别处死模型组大鼠4只、5只,余大鼠均于第12周末处死,观察各模型组大鼠一般情况、肝脏指数、光镜下肝组织损伤程度,分别用速率法、GPO—PAP法检测血清天门冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)、血清总胆固醇(TC)和甘油三酯(TG)水平;用TBA比色分析法测定12周末大鼠肝组织丙二醛(MDA)水平、黄嘌呤氧化酶(XO)活性。结果:模型组大鼠一般情况较中、西药组差,肝湿重、肝指数有逐渐增加趋势(P均>0.05),血清ALT、AST、TC、TG水平均呈明显上升趋势(P均<0.05),HE染色光镜下观察肝组织呈不同程度脂肪变性;与模型组比较,中、西药组大鼠肝组织脂肪变明显较轻;大鼠肝湿重、肝指数,中、西药组较模型12周组及中药组较模型8周组均有下降趋势(P均>0.05);中、西药组大鼠分别较模型8周、12周组大鼠血清ALT、AST、TC、TG水平明显降低(P均<0.05),较模型4周组大鼠血清ALT、AST、TC水平明显降低(P均<0.05);与模型组12周相比,中、西药组大鼠肝组织匀浆MDA含量及XO活性明显下降(P<0.05);中、西药组大鼠肝湿重和肝指数,血清ALT、AST、TC、TG水平,肝组织匀浆MDA含量及XO活性差异无统计学意义(P>0.05)。结论:采用高脂饮食联合酒精灌胃的方法可成功诱导SD大鼠AFLD模型;益肝降脂方药可明显减轻AFLD大鼠肝组织脂肪变、炎症程度及病理损伤并显示出时、效相应的特点,可能是早期有效干预治疗AFLD的作用机制之一。
Objective: To observe the effect of Yiganghua recipe on early intervention in rats with alcoholic fatty liver disease (AFLD). Methods: Totally 85 male Sprague-Dawley rats were randomly divided into model group (n = 25), Chinese medicine group (n = 30) and western medicine group (n = 30). AFLD model was established by intragastric administration of high fat diet combined with gradient liquor for 12 weeks. Saline, Yigan lipid-lowering herbs and easy to reconcile suspension; at the end of the 4th and 8th weeks, 4 rats in the model group were sacrificed, and 5 rats were sacrificed at the end of the 12th week. The rats in each model group were generally (P <0.05). The serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), serum total cholesterol ) And triglyceride (TG). The level of malondialdehyde (MDA) and the activity of xanthine oxidase (XO) in rat liver tissue were measured by TBA colorimetry. Results: The rats in the model group showed a general increase in the levels of ALT, AST, TC and TG (P <0.05, P <0.05) 0.05). Hepatic steatosis was observed by HE staining under light microscope. Compared with the model group, the fatty changes of liver tissue in rats in the Chinese and western medicine groups were lighter than those in the model group. The liver wet weight, liver index, The levels of ALT, AST, TC and TG in serum of rats in Chinese and western medicine groups were significantly lower than those of the model group at 8 and 12 weeks respectively (P> 0.05) P <0.05). Compared with the model group, the levels of ALT, AST and TC in serum in the model group decreased significantly (P <0.05) (P <0.05). There was no significant difference in liver wet weight, liver index, serum ALT, AST, TC, TG levels, liver MDA content and XO activity in the Western medicine group (P> 0.05). Conclusion: The method of high-fat diet combined with alcohol gavage can successfully induce AFLD model in SD rats. Yiganheng lipid-lowering prescription can obviously reduce hepatic steatosis, inflammation and pathological damage in AFLD rats, It may be one of the mechanisms of effective early intervention in the treatment of AFLD.