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为了了解导入Bt基因是否会引起玉米对磷元素利用能力发生改变,本研究采用转基因抗虫玉米Bt 38及其背景材料郑单958(ZD 958)为试验材料,进行水培试验,设置正常供磷(1mmol/L)和低磷(1μmol/L)两个水平。结果表明:在相同磷浓度下,ZD 958与Bt 38的某些生物性状、生理性状显著地不同,在生物性状上,正常供磷条件下与ZD 958相比,Bt 38根长极显著降低,全展叶叶面积极显著减小,低磷条件下与ZD 958相比,Bt 38株高、根长、茎粗、叶长,叶宽、地上鲜重、地下鲜重均极显著增加,根冠比显著下降;在生理特征上,相同供磷条件下,与ZD 958相比,抗虫玉米心叶、全展叶叶绿素含量及Chla/Chlb均有显著性变化,抗虫玉米不同部位可溶性糖、淀粉含量、激素含量亦有显著性变化。此外,全展叶中可溶性蛋白含量和抗氧化物酶活性也有显著性变化。综上,Bt基因的导入改变了ZD 958苗期对磷元素的利用能力,从而使ZD 958与Bt 38在不同磷环境下表现出不同的生存能力。
In order to understand whether the introduction of Bt gene will cause changes in the ability of maize to use phosphorus, transgenic Bt 38 transgenic rice and its background material Zhengdan 958 (ZD 958) were used as materials to conduct the hydroponics experiment. (1mmol / L) and low phosphorus (1μmol / L) two levels. The results showed that under the same phosphorus concentration, some biological and physiological traits of ZD 958 and Bt 38 were significantly different. Compared with ZD 958, the root length of Bt 38 significantly decreased under the normal phosphorus supply, Compared with ZD 958, Bt 38 plant height, root length, stem diameter, leaf length, leaf width, aboveground fresh weight and underground fresh weight all increased significantly under low phosphorus condition. Crown ratio decreased significantly. Compared with ZD 958, chlorophyll content and Chla / Chlb in the resistant and susceptible maize leaves had significant changes on the physiological characteristics and in the same phosphorus supply, soluble sugar , Starch content, hormone content also had significant changes. In addition, soluble protein content and antioxidant enzyme activity also showed a significant change in the whole leaf. In summary, the introduction of Bt gene changed the ability of ZD 958 to utilize phosphorus element at the seedling stage, which made ZD 958 and Bt 38 show different viabilities in different phosphorus environments.