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以产孢量大,对苯菌灵有抗性,对潮霉素B敏感的哈茨木霉菌株T9和产孢量少,对潮霉素B有抗性,对苯菌灵敏感的康宁木霉菌株Tk7a为亲本,进行原生质体融合,筛选获得抗最高浓度杀菌剂的融合子。所有融合子的产孢量均比亲本菌株Tk7a大。融合子按照菌落形态的不同分成4组,大部分融合子的几丁质酶、葡聚糖酶和内切葡聚糖酶的活性比亲本菌株高。融合子对棉花猝倒病菌和小麦全蚀病菌的寄生能力基本等同于亲本菌株;融合子对小麦全蚀病的防效比亲本菌株稍好,其刺激麦苗根部生长的能力与亲本菌株相差不大。两个融合子根际竞争能力比亲本菌株T9强,和亲本菌株Tk7a相当。
In order to sporulation large, resistant to benomyl, hygromycin B-sensitive strains of Trichoderma harzianum T9 and less spores, hygromycin B resistance to benomyl-sensitive Coronatine Strain Tk7a as the parent, protoplast fusion, screened to obtain the highest concentration of fungicide fusants. The sporulation amount of all the fusants was larger than that of the parental strain Tk7a. The fusants were divided into four groups according to the colony morphology. The chitinase, glucanase and endoglucanase of most of the fusants were more active than the parent strain. The results showed that the fusion ability of the fusion gene to cotton bollworm and wheat total ectomycorrhizal fungi was basically the same as that of the parental strain. The control effect of the fusion plant on wheat ecloggenosis was slightly better than that of the parental strain, and its ability of stimulating the root growth of wheat seedlings was similar to that of the parent strain . The two fusants were more competitive in the rhizosphere than the parental strain T9 and comparable to the parental strain Tk7a.