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在各种评价精子膜功能和形态学完整性的检测中,一种较常用的方法是基于有膜损伤的死精子能吸收超活体染料,如Eosin—Y;而活细胞却不能被染色。当不活动的死精子膜损伤很轻微,不足以透过Eosin—Y 分子时,就难以与不活动而活着的精子相区分。Heyendran 等提出用低渗溶液使人精子肿胀后可在相差显微镜下观察人精子膜功能完整性。低渗溶液由150毫渗的果糖和柠檬酸钠组成,离子强度为0.15。但是已有研究发现渗透压相同的溶液可能对膜有不同的影响。糖和电解质对水通过
One of the more common methods used to test sperm membrane function and morphological integrity is based on the fact that dead sperm with membrane damage can absorb superviable dyes such as Eosin-Y, whereas living cells can not be stained. When inactive dead sperm membrane damage is very slight, not enough to penetrate the Eosin-Y molecules, it is difficult to distinguish between inactive and inactive sperm. Heyendran et al. Proposed that hypotonic human sperm can be swollen with hypotonic solution to observe the functional integrity of human sperm membrane under a phase-contrast microscope. The hypotonic solution consists of 150 milliliters of fructose and sodium citrate with an ionic strength of 0.15. However, studies have found that the same solution of osmotic pressure may have different effects on the membrane. Sugar and electrolytes pass through the water