【摘 要】
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Dear Editor,RUB/Nedd8 (RELATED TO UBIQUITIN/neuronal precursor cell-expressed developmentally downregulated 8) is a 76-amino acid protein that modifies other pr
【机 构】
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Molecular and Cellular Biology,University of California,Davis,CA 95616,USA“,”Proteomics Core Facilit
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Dear Editor,RUB/Nedd8 (RELATED TO UBIQUITIN/neuronal precursor cell-expressed developmentally downregulated 8) is a 76-amino acid protein that modifies other proteins and has homology to UBIQUITIN (UBQ).Like UBQ,RUB modifies proteins through an isopeptide linkage between its C-terminal carboxyl group and the ε-amino group of a lysine in the acceptor protein and attachment occurs via an E1-E2-E3 enzymatic cascade.RUB is known to modify a limited pool of proteins,primarily members of the cullin (CUL) family that function in CUL-RING (CRL) E3 ligases.The cullins,CUL1,CUL3a/b,and CUL4,are the only confirmed RUB-modified proteins in plants (Hua and Vierstra,2011),although additional substrates have been identified in non-plant systems (Rabut and Peter,2008).
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