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采用ELISA间接夹心法测定了23名成人牙周炎患者34个患牙龈沟液中的IL-8.并用免疫组织化学法对相应的炎性龈组织进行检测.结果表明IL-8与牙周炎症密切相关,牙周炎龈沟液(GCF)中IL-8检出率为88.24%.IL-8含量范围为0.006～2.772ng/μl.其含量与牙龈指数相关非常显著,与牙周袋深度相关显著,与附着丧失、菌班指数无明显相关.炎性龈组织切片染色表明龈上皮组织和胶原组织中含有大量的阳性染色,从组织学上证明了GCF中IL-8的来源.提示IL-8可能在牙周炎发病机制中起着重要作用.
IL-8 in gingival crevicular fluid of 34 adult patients with periodontitis was detected by indirect ELISA sandwich ELISA, and the corresponding inflammatory gingival tissues were detected by immunohistochemistry.The results showed that IL-8 and periodontal inflammation Closely related to the detection of IL-8 in gingival crevicular fluid (GCF) of periodontitis was 88.24% and the content of IL-8 was 0.006 ~ 2.772ng / μl.The content of IL-8 was significantly correlated with gingival index and significantly correlated with the depth of periodontal pocket , Which showed no significant correlation with the loss of adhesion and the index of bacteria class.The inflammatory gingival tissue sections showed a large number of positive staining in the gingival epithelium and collagen and the origin of IL-8 in GCF was confirmed histologically It may play an important role in the pathogenesis of periodontitis.