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目的：通过测定葡萄糖－６－磷酸脱氢酶（Ｇ６ＰＤ）缺乏红细胞膜磷脂的含量及其不对称性的变化，研究Ｇ６ＰＤ缺乏对红细胞膜结构的影响。方法：不对称性实验采用磷脂酶Ａ２处理完整红细胞，磷脂的测定采用高效液相色谱法。结果：正常红细胞膜磷脂酰乙醇胺（ＰＥ）、磷脂酰丝氨酸（ＰＳ）、磷脂酰胆碱（ＰＣ）含量分别为５．５９±１．１６，２．９２±０．５０，６．６４±０．９２ｍｇ／ｍｇ膜蛋白；Ｇ６ＰＤ缺乏红细胞膜ＰＥ、ＰＳ、ＰＣ分别为５．１８±１．８６，１．５１±０．５８，５．７０±１．９１ｍｇ／ｍｇ膜蛋白，两组中ＰＳ有显著性差异（Ｐ＜０．００１），说明Ｇ６ＰＤ缺乏红细胞膜ＰＳ含量降低。在正常人红细胞膜中有１７．６％±２．５％ＰＥ，５７．９％±４．７％ＰＣ在外层，外层未发现ＰＳ，在Ｇ６ＰＤ缺乏红细胞膜中有３０．２％±５．７％ＰＥ，５３．８％±７．４％ＰＣ，２７．２％±５．８％ＰＳ在外层，两组中ＰＥ和ＰＳ有显著性差异（Ｐ＜０．０１和Ｐ＜０．００１），外层的ＰＣ无明显变化，说明ＰＥ、ＰＳ均向外层移动。结论：由于Ｇ６ＰＤ缺乏，红细胞膜磷脂结构发生改变，是红细胞溶血的重要机制之一。
OBJECTIVE: To investigate the effect of G6PD deficiency on erythrocyte membrane structure by measuring the content of phosphoglycerate-6-phosphate dehydrogenase (G6PD) deficiency and its asymmetry. Methods: Asymmetry experiments using phospholipase A2 treatment of intact red blood cells, phospholipids measured using high performance liquid chromatography. Results: The content of phosphatidylethanolamine (PE), phosphatidylserine (PS) and phosphatidylcholine (PC) in normal erythrocyte membrane were 5.59 ± 1.16,2.92 ± 0.50,6.64 ± 0 .92mg / mg membrane protein; G6PD lack of erythrocyte membrane PE, PS, PC were 5.18 ± 1.86,1.51 ± 0.58,5.70 ± 1.91mg / mg membrane protein, PS There was a significant difference (P <0.001), indicating a lack of G6PD erythrocyte membrane PS content decreased. There were 17.6% ± 2.5% PE in normal human erythrocyte membrane, 57.9% ± 4.7% PC in outer layer, no PS in outer layer, 30.2% ± 5 in G6PD-deficient erythrocyte membrane .7% PE, 53.8% ± 7.4% PC and 27.2% ± 5.8% PS in the outer layer. There was a significant difference between PE and PS in both groups (P <0.01 and P <0. 001), the outer PC no significant change, indicating PE, PS are moving to the outer layer. Conclusion: Due to the lack of G6PD, the phospholipid structure of erythrocyte membrane is changed, which is one of the important mechanisms of hemolysis of erythrocytes.