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采用改进的异硫氰酸胍－酚－氯仿一步抽提法提取肠道病毒ＲＮＡ，通过反复试验，建立了单管一ＲＴ一ＰＣＲ（Ｒｅｖｅｒｓｅｔｒａｎｓｃｒｉｐｔｉｏｎｐｏｌｙｍｅｒａｓｅｃｈａｉｎｒｅａｃｔｉｏｎ）反应体系。结果表明，此法较常规的分步法简便快速、可靠，为临床检测和流行病学研究提供了较理想的方法。
An improved one-step extraction of guanidine isothiocyanate-phenol-chloroform was used to extract enteroviral RNA. Through repeated experiments, a one-tube-one RT-PCR (Reversetranscription polymerase chain reaction) reaction system was established. The results show that this method is simpler, faster and more reliable than the conventional one-step method, which provides an ideal method for clinical testing and epidemiological studies.