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With high association constants in nonpolar solvents, the quadruple hydrogen bonding arrays of 2-ureido-4[1H]-pyrimidinones are recently considered to be one of the ideal building blocks to construct the complicated and functional supramole- cular systems. In this paper, we review the latest achievements of the supramolecular assemblies based on 2-ureido-4[1H]-pyrimidinone AADD quadruple hydrogen bonding building blocks.