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以阿司匹林为内标,在YWG-CN柱上用CH_3CN:CH_3COONa-H_2O:CHCl_3(10:90:1)为流动相用反相HPLC法测定了毫州芍药(毛果芍药P.lactiflora var.trichocarpa)不同物侯期根中羟基芍药甙(Ⅰ)、芍药内酯甙(Ⅱ)、芍药甙(Ⅲ)和苯甲酸(Ⅳ)的含量。结果发现,毫州地区毛果芍药根中甙类成分的产量在9月中旬呈最高水平;Ⅱ与Ⅲ的含量相关性较好(r=0.7848),在生合成上有某些内在联系;高温、少雨对甙类成分的积累不利。
Aspirin was used as the internal standard and the HPLC method was used for the determination of carrageenan (P.lactiflora var.trichocarpa) on the YWG-CN column using CH_3CN:CH_3COONa-H_2O:CHCl_3 (10:90:1) as the mobile phase. ) Contents of hydroxy phthalein (I), peony lactone (II), peony glycosides (III), and benzoic acid (IV) in roots from different periods. The results showed that the production of anthraquinones in the medicinal roots of Fructus americana was the highest in mid-September; the correlation between the contents of II and III was good (r=0.7848), and there was some intrinsic connection in the biosynthesis. The lack of rain has adversely affected the accumulation of terpenoids.