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慢阻肺在老年人发病率高,由于反复住院及应用抗生素,使院内绿脓杆菌(PA)肺感染增加,且治疗困难,本文主要从PA分型、药敏及临床疗效等方面对诊疗方法进行探讨。 1 材料与方法 1.1 病例来源:均为住院患者,共60例,男34例,女26例,年龄均大于60岁,其中慢性支气管炎12例,肺气肿28例,肺心病20例。 1.2 PA分离培养及分型:对60例患者做痰定量培养,并结合冷休克试验确定培养所得60株PA为院内肺感染致病菌,对60株PA进行联合分型。血清学分型用卫生部成都生物制品研究所研制的20型一套诊断血清,采用玻片凝集法;噬菌体分型用江
Chronic obstructive pulmonary disease in the elderly with high incidence, due to repeated hospitalizations and the use of antibiotics to make the hospital Pseudomonas aeruginosa (PA) increased pulmonary infection, and the treatment is difficult, this article mainly from PA typing, drug sensitivity and clinical efficacy of the treatment methods To explore. 1 Materials and Methods 1.1 Case Source: All inpatients, a total of 60 cases, 34 males and 26 females, more than 60 years of age, including chronic bronchitis in 12 cases, emphysema in 28 cases, 20 cases of pulmonary heart disease. PA isolation and culture and typing: 60 patients were sputum quantitative culture, combined with cold shock test to determine the culture of 60 PA obtained for nosocomial pulmonary pathogens, 60 PA were typed. Serological typing with the Ministry of Health, Chengdu Institute of Biological Products developed a set of 20 diagnostic serum, using glass agglutination method; phage typing with Jiang