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建立了测定麻疹病毒抗体的酶免疫电化学分析法。其原理是在异相酶联免疫之后用极谱法检测酶促产物。本法采用小型三电极系统“ＤＭＥ－Ｐｔ－Ａｇ／ＡｇＣｌ”直接与商品酶联板配套使用，简化了操作步骤。对测定麻疹抗体的合适条件以及灵敏度，特异性等作了考察。结果表明，本法特异性强，灵敏度高，检测范围宽，不受样品颜色、浊度、杂散光及酶联板自身光吸收不均一的影响；应用于测定三批共１１８份血清样品，阳性率分别为１５％（６／４０），６２．５％（２５／４０）和３１．５８％（１２／３８），用４６份样品对本法和ＥＬＩＳＡ作相关性检验，其相关性良好（γ＝０．７７．ｔ＿ｒ＝１０．８４，Ｐ＜０．００１，相关显著）。
An enzyme immuno-electrochemical assay for the determination of measles virus was established. The principle is the use of polarographic detection of enzymatic products after heterogeneous enzyme-linked immunosorbent assay. This method uses a small three-electrode system “DME-Pt-Ag / AgCl” directly with the product of enzyme-linked board supporting the use of simplified steps. Appropriate conditions for the determination of measles antibodies, as well as sensitivity, specificity were investigated. The results showed that this method has the characteristics of strong specificity, high sensitivity and wide detection range. It is not affected by the uneven absorption of sample light, turbidity, stray light and enzyme-linked plate itself. It is suitable for the determination of 118 serum samples in three batches, The rates were 15% (6/40), 62.5% (25/40) and 31.58% (12/38), respectively. The correlation between this method and ELISA was good with 46 samples (γ = 0.77.t_r = 10.84, P <0.001, correlated significantly).