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研究了脊尾白虾(Exopalaemon carinicauda)在对苯并[a]芘(benzo[a]pyrene,Ba P)的富集(15 d)、释放(15 d)过程中肝脏的生物标志物(EROD、GST、SOD、CAT、GPx、MDA)的响应,结果显示:在富集阶段,第1 d低浓度实验组(0.05μg/L)和高浓度实验组(0.45μg/L)六种生物标志物即均显著受到诱导(P<0.05),诱导程度与暴露浓度成正相关,而后不同种类生物标志物呈现差异性的变化趋势,EROD继续上升并在第10 d后达到稳定状态,GST继续上升并在第10 d后受到抑制下降,SOD、CAT、GPx均受到抑制逐渐下降,且均在15 d时低于对照组水平,MDA呈现持续上升的趋势,直接反映了肝脏的氧化损伤程度。在释放阶段,低浓度实验组各生物标志物的恢复速度较高浓度实验组要快,且均能恢复到接近对照组水平(P>0.05),高浓度实验组各生物标志物则均未能恢复到对照组水平(P<0.05)。实验结果表明,脊尾白虾肝脏具有一定恢复能力,各生物标志物对Ba P暴露的敏感性具有一定差异,Ba P暴露的浓度及时间是影响各生物标志物响应变化的主要因素。
The effects of enrichment of benzo [a] pyrene (BaP) on Exopalaemon carinicauda (15 d) and biomarkers of liver release (EROD, GST, SOD, CAT, GPx and MDA). The results showed that in the enrichment stage, the six low-concentration test groups (0.05μg / L) and the high concentration test groups (0.45μg / L) (P <0.05). The degree of induction was positively correlated with the exposure concentration, and then the different types of biomarkers showed a trend of variation. EROD continued to rise and reached a steady state after 10 days, and GST continued to rise After the 10th day, the inhibition decreased, and the SOD, CAT and GPx decreased gradually. Both of them were lower than the control group on the 15th day. The MDA showed a rising trend, which directly reflected the degree of oxidative damage in the liver. During the release phase, the biomarkers of low concentration group had faster recovery than those of the control group (P> 0.05), and none of the biomarkers of the high concentration group failed to recover Recovery to the control group level (P <0.05). Experimental results show that the liver of Pleurotus intermedius has a certain ability to recover, the sensitivity of biomarkers to exposure to Ba P has some differences, the concentration of Ba P exposure and time are the main factors that affect the response of each biomarker.