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本文报道棉酚对睾丸线粒体功能及超微结构的影响,并根据实验结果提出棉酚作用机理假说。 电镜观察证明生精细胞线粒体是对棉酚最敏感和损伤最严重的细胞器,呈现由肿胀、空泡化、嵴突缺损、溶解,到整体崩解等损伤过程。精子线粒体特异的乳酸脱氢酶同功酶(LDH-X)在服棉酚后明显受到抑制。 用Warburg呼吸器和Clark氧电极测定结果表明棉酚到达一定浓度后可完全抑制离体睾丸线粒体的呼吸(300μM)和氧化磷酸化(80μM)过程,其解偶联作用与剂量大小成正相关。大鼠口服棉酚后分离的睾丸线粒体的P/O值亦同样呈现非常明显的降减。 ~(14)C-棉酚对精子线粒体有较高亲和性,其分布量较其他亚细胞组分高2—3倍.与~(3)H-哇巴因一起示踪定位亦得到类似的结果。 对棉酚的亚细胞作用位点及其中断精子发生的分子作用机理以及不同细胞类型对棉酚的敏感性等问题进行了分析和讨论。
This article reports the effects of gossypol on the function and ultrastructure of testicular mitochondria, and based on the experimental results suggest that the mechanism of gossypol hypothesis. Electron microscopy showed that spermatogenic cell mitochondria were the most sensitive and injured cells to gossypol, and showed the processes of swelling, vacuolization, crista defects, dissolution, and overall disintegration. Sperm mitochondria-specific lactate dehydrogenase isoenzyme (LDH-X) after serving gossypol was significantly inhibited. The results of Warburg respirator and Clark oxygen electrode showed that gossypol could completely inhibit the respiration (300μM) and oxidative phosphorylation (80μM) of isolated testicular mitochondria after reaching a certain concentration, and the uncoupling effect was positively correlated with the dose size. The P / O values of testicular mitochondria isolated from rats after oral administration of gossypol also showed a very significant reduction. ~ (14) C-gossypol has high affinity to sperm mitochondria, which is 2-3 fold higher than that of other subcellular components. Similar tracking with ~ (3) H-ouabain was also found the result of. The subcellular location of gossypol and its mechanism of molecular action in interrupting spermatogenesis and the sensitivity of different cell types to gossypol were analyzed and discussed.