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目的探讨甘油二酯(DAG)对糖尿病肾病SD大鼠肾组织蛋白激酶C(PKC)表达和氧化应激的影响。方法SD雄性大鼠40只,选10只为正常对照组(I)用基础饲料喂养。另30只,采用高脂高糖饲料喂养结合链脲佐菌素(STZ)注射诱导2型糖尿病肾病模型(2型DN)。DN成模20只,均分为甘油三酯组(TAG,II)和甘油二酯两组(DAG,III)分别用TAG、DAG饲料持续喂养8w两组饲料组分(%)除TAG、DAG不同外,其余均相同,为:酪蛋白纤维素粉4,矿物质混合物3.5,维生素混合物1,淀粉61.5。实验第2、4、6、8w分别检测空腹血糖(FBG);实验结束前检测24h尿蛋白(UPRO)、血肌酐(SCR);采用苏木精-伊红(HE)染色和Massion染色观察肾脏的病理变化;采用试剂盒法检测肾皮质丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性、谷胱甘肽过氧化酶(GSH)活性;免疫组化法检测肾脏中PKC的表达。结果与II组比较,III组可以显著降低模型大鼠FBG(P<0.05),UPRO排泄率显著减少(P<0.05),MDA含量显著下降(P<0.05),肾组织病理改变减轻,并且肾组织内PKC表达下调(P<0.05)。结论 DAG能改善DN大鼠肾功能,抑制氧化应激反应,下调PKC表达,延缓DN的发生发展;推测DAG保护2型糖尿病大鼠肾脏的机制与其有关。
Objective To investigate the effect of diglyceride (DAG) on protein kinase C (PKC) expression and oxidative stress in the kidney of SD rats with diabetic nephropathy. Methods Forty male Sprague-Dawley rats were randomly divided into normal control group (I) and basal diet. The other 30 rats were fed with high-fat and high-sugar diet combined with streptozotocin (STZ) injection to induce type 2 diabetic nephropathy model (type 2 DN). DN were divided into two groups (TAG, II) and diglyceride (DAG, III). TAG and DAG were respectively fed to the two groups for 8 weeks (TAG, DAG) Different, the rest are the same as: casein cellulose powder 4, mineral mixture 3.5, vitamin mixture 1, starch 61.5. Fasting blood glucose (FBG) was measured at the 2nd, 4th, 6th and 8th weeks after the experiment. UPRO and serum creatinine (SCR) were measured before the experiment. The kidneys were observed by hematoxylin and eosin (HE) staining and Massion staining The contents of malondialdehyde (MDA), superoxide dismutase (SOD) and glutathione peroxidase (GSH) in renal cortex were determined by kit method. The expressions of PKC in kidney were detected by immunohistochemistry expression. Results Compared with group II, group III significantly reduced the FBG (P <0.05), the rate of UPRO excretion (P <0.05), the content of MDA decreased significantly (P <0.05), and the pathological changes of kidney decreased The expression of PKC in the tissue was down-regulated (P <0.05). Conclusion DAG can improve renal function, inhibit oxidative stress, decrease PKC expression and delay the development of DN in DN rats. It is speculated that DAG protects the kidney of type 2 diabetic rats.