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目的研究天然酮类化合物抗柯萨奇病毒B5(CVB5)的作用及其机理。方法通过改变给药途径,观察病毒引起的细胞病变效应(CPE),MTT法检测细胞活性,评价酮化合物在Hep-2细胞培养中的抗CVB5作用。结果酮具有抗CVB5的活性,对Hep-2细胞的TC50为54.44μg·mL-1,其EC50分别为26.378、25.813、25.405μg·mL-1,治疗指数(TI)分别为2.064、2.109、2.143;在32μg·mL-1时,对CVB5的抑制率达50%。结论酮化合物通过直接杀灭病毒、阻止病毒的吸附、抑制病毒的生物合成而发挥其抗CVB5的作用;但需对结构做进一步的修饰,以降低其毒性。
Objective To study the effect of natural ketones on Coxsackievirus B5 (CVB5) and its mechanism. Methods The cytopathic effect (CPE) caused by virus was observed by changing the route of administration. The activity of CVB5 in Hep-2 cells was evaluated by MTT assay. Results Chlordone had anti-CVB5 activity. The TC50 of Hep-2 cells was 54.44μg · mL-1 with EC50 of 26.378,25.813 and 25.405μg · mL-1, respectively. The therapeutic indices (TI) were 2.064 and 2.109, 2.143; At 32μg · mL-1, the inhibition rate of CVB5 reached 50%. Conclusions Chironone compounds exert their anti-CVB5 effect by directly killing the virus, preventing the adsorption of the virus and inhibiting the biosynthesis of the virus; however, the structure needs to be further modified to reduce its toxicity.