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取培养 1 2天的神经元 ,置于缺氧环境中培养 4小时后取出 ,再置含 0 .1 0Ｌ ＬＣＯ2 和空气培养箱内复氧培养 2 4小时和 72小时 ,于不同时间取出 ,观察其存活数、Ｊｕｎ表达阳性和阴性的神经元数。结果发现缺氧前对照组和低氧预处理组神经元存活数未见明显变化 ;缺氧前两组海马
Neurons were harvested for 12 days. The cells were placed in anoxic environment for 4 hours and then removed. The cells were re-incubated with 0.1 1 L of LCO2 and reoxygenated in an air incubator for 24 hours and 72 hours. The cells were removed at different times and observed Survival number, Jun expression positive and negative neurons. The results showed that the number of neurons survived in hypoxic preconditioning and hypoxic preconditioning groups showed no significant changes; the hippocampus