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目的建立小艾飞蜜膏(荜茇、高良姜)的质量分析方法。方法采用薄层色谱法(TLC)对小艾飞蜜膏中的胡椒碱和高良姜素进行定性鉴别;采用高效液相色谱法(HPLC)分别测定胡椒碱、高良姜素的含量。结果薄层色谱鉴别均斑点清晰,阴性对照无干扰;定量测定中胡椒碱在24~144μg浓度范围内与峰面积呈良好线性关系(r=0.999 9),平均回收率为100.09%,RSD为1.04%(n=6)。高良姜素在2.16~12.96μg浓度范围内与峰面积呈良好线性关系(r=0.999 9),平均回收率为100.06%,RSD为1.17%(n=6)。结论所建立的方法操作简单,专属性和重复性良好,可作为该制剂的质量控制方法之一。
Objective To establish the method of quality analysis of Xiaoyi honey cream (Gorgon, galangal). Methods TLC and HPLC were used to identify piperine and galangin in Xiaoyi honey cream. The contents of piperine and galangin in the samples were determined by high performance liquid chromatography (HPLC). Results The results of TLC showed that the spots were clear and the negative control had no interference. The quantitative determination of piperine showed a good linear relationship with the peak area in the range of 24-144 μg (r = 0.999 9), with an average recovery of 100.09% and a RSD of 1.04 % (n = 6). Galangin had a good linear relationship with the peak area in the range of 2.16 ~ 12.96μg (r = 0.999 9), with an average recovery of 100.06% and a RSD of 1.17% (n = 6). Conclusion The established method is simple, specific and reproducible, which can be used as one of the quality control methods for this preparation.