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目的建立一种新的石墨炉原子吸收法来快速测定全血中的铝。方法向200μl全血样品中加入800μl混合处理液(含40 ml/L硝酸与6 ml/L过氧化氢),静置20 min后,4℃条件下,12000 r/min离心6 min,离心分离脱去全血中的蛋白,以3.0 g/L硝酸镁为化学改进剂,上清液用石墨炉原子吸收光谱法直接测定。结果在铝的最大吸收波长309.3 nm下,血液中铝含量在10μg/L~250μg/L范围内线性良好,相关系数r=0.9998。此方法的最低检出限为3μg/L,加标回收率为92.5%~110.0%,相对标准偏差为(RSD)为1.9%~9.7%。结论方法已成功应用于33例血透病人全血样品中铝的测定,结果令人满意。
Objective To establish a new graphite furnace atomic absorption spectrometry for the rapid determination of aluminum in whole blood. Methods 200 μl of whole blood samples were mixed with 800 μl of mixed solution (containing 40 ml / L nitric acid and 6 ml / L hydrogen peroxide), allowed to stand for 20 min, centrifuged at 12000 r / min for 6 min at 4 ° C and centrifuged Remove the whole blood of the protein to 3.0 g / L of magnesium nitrate as a chemical modifier, the supernatant was directly determined by graphite furnace atomic absorption spectrometry. Results The aluminum concentration in the blood ranged from 10μg / L to 250μg / L at a maximum absorption wavelength of 309.3 nm. The correlation coefficient was 0.9998. The detection limit of this method was 3μg / L, the recoveries were 92.5% ~ 110.0%, and the relative standard deviations (RSDs) were 1.9% ~ 9.7%. Conclusion The method has been successfully applied to the determination of aluminum in 33 hemodialysis patients with satisfactory results.