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为了解害虫个体的迁移对害虫群体抗药性发展的影响,本研究利用实验室内建立的桔小实蝇Bactrocera dorsalis对敌百虫和高效氯氰菊酯两种抗性品系以及相对敏感品系,设计5%～25%个体的迁移比例,研究桔小实蝇抗、感个体流动对原始种群抗性个体频率的影响。结果表明:桔小实蝇抗性个体迁入敏感种群,使得敏感种群抗性个体频率增加,在抗性个体迁移率为25%时,影响敏感种群敌百虫和高效氯氰菊酯抗性个体频率的变化值分别为20.04%和41.75%;同样随着敏感个体迁入比例的增加,抗性种群中抗性个体频率降低程度越大,在敏感个体迁移率为25%时,抗性种群敌百虫和高效氯氰菊酯抗性个体频率的变化值分别为56.20%和25.88%。利用抗性个体频率变化值与相应迁移率的比值来表示迁移的相对效率,在抗性个体迁移率为5%时,影响敏感种群抗性个体频率变化的相对效率最高;在敏感个体迁移率分别为5%和10%时,影响敌百虫抗性种群和高效氯氰菊酯抗性种群抗性个体频率变化的相对效率最高。以抗、感个体迁移引起的抗性个体频率变化值进行趋势拟合,发现抗敌百虫与抗高效氯氰菊酯桔小实蝇品系不同迁移比例下的抗性个体频率变化趋势分别符合密度模型(density model)和房屋模型(housing model),相关系数分别是0.9696和0.9647。研究结果表明通过合理地设计抗、感迁移比例能有效地延缓桔小实蝇抗性水平的上升,达到抗性治理的目的。
In order to understand the impact of individual pest migration on the development of drug resistance in pest populations, the present study established a laboratory-established Bactrocera dorsalis Trichogramma and cypermethrin two resistant strains and relatively sensitive strains designed 5% 25% of the individual migration ratio, to study the impact of anti-dipsacus and susceptible individuals on the frequency of resistant individuals in the original population. The results showed that the resistance population of susceptible population of immigrant fruit fly migrated into sensitive population increased the frequency of resistant individuals in susceptible population, and affected the frequency of susceptible population trichlorfon and beta-cypermethrin when the migration rate of resistant individuals was 25% The values were 20.04% and 41.75%, respectively. Also with the increase of the immigrating proportion of sensitive individuals, the decreasing frequency of resistant individuals in resistant populations was greater. When the mobility of sensitive individuals was 25% The change of beta-cypermethrin-resistant individuals was 56.20% and 25.88% respectively. The relative efficiency of migration was expressed by the ratio of the frequency of the resistant individuals and the corresponding mobility. When the mobility of the resistant individuals was 5%, the relative efficiency of the frequency of resistance individuals with sensitive populations was the highest. In the sensitive individuals, Was 5% and 10%, respectively, the relative efficiency of the frequency of individuals affected by the trichlorfon-resistant population and the beta-cypermethrin-resistant population was the highest. The results showed that the frequency of resistance individuals at different migration rates of trichlorfon and beta-cypermethrin crosses were in accordance with the density model (density model and housing model, the correlation coefficients are 0.9696 and 0.9647, respectively. The results show that the rational design of anti-sense transfer ratio can effectively delay the rise of resistance level of the fruit fly to achieve the purpose of resistance management.