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目的了解镇海区手足口病病人和密切接触者病毒携带及毒株动态变化,为手足口病防控提供实验室依据。方法用荧光定量PCR方法检测手足口病病人及其密切接触者粪便中肠道通用型病毒、EV71和CA16核酸。结果 2011年和2012年病人和密切接触者肠道通用病毒核酸检出率分别为70.31%、61.902%和35.06%、28.03%,无统计学意义差异(P>0.05)。2011年EV71核酸检出率为27.98%(61/218),CA16核酸检出率为14.22%(31/218),2012年EV71核酸的检出率为5.45%(12/220),CA16核酸的检出率为20.45%(45/220),两者均有统计学意义差异(P<0.05)。结论 2011年感染以EV71为主,2012年则以感染CA16为多,存在毒株交替发生现象;第2季、3季度为发病的高峰期,与疾病的流行规律一致。
Objective To understand the dynamic changes of virus carriers and strains in hand, foot and mouth disease patients and close contacts in Zhenhai District, and provide a laboratory basis for prevention and control of hand, foot and mouth disease. Methods Fluorescent quantitative PCR was used to detect enterovirus, EV71 and CA16 nucleic acids in feces of HFMD patients and their close contacts. Results The detection rates of intestine universal nucleic acid in patients and close contacts in 2011 and 2012 were 70.31%, 61.902% and 35.06%, 28.03%, respectively. There was no significant difference (P> 0.05). In 2011, the detection rate of EV71 nucleic acid was 27.98% (61/218), the detection rate of CA16 nucleic acid was 14.22% (31/218), the detection rate of EV71 nucleic acid was 5.45% (12/220) in 2012, The detection rate was 20.45% (45/220), both of which had statistical significance (P <0.05). Conclusion In 2011, EV71 was predominant, while in 2012, CA16 was the most frequently infected strain. Alternations occurred in the second quarter and the third quarter, which was consistent with the prevalence of the disease.