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目的探讨EBV感染引发IM与CAEBV患儿的外周血淋巴细胞亚群及免疫功能之间的差异,为临床诊断提供可靠的数据支持。方法回顾分析郑州市儿童医院儿科40例EBV患儿临床资料,IM患儿21例,CAEBV患儿19例;选择同期健康儿童20例。采用流式细胞仪检测外周血血常规及淋巴细胞亚群;使用比浊法检测血清免疫球蛋白水平。观察比较3组外周血血常规及淋巴细胞亚群检测结果、免疫球蛋白检测结果。结果三组外周血血常规及淋巴细胞亚群检测结果差异有统计学意义(P<0.05);其中,IM组患儿的外周血血常规检测结果及淋巴细胞亚群检测中的CD~+3T、CD~+4T、CD~+8T计数高于CAEBV组、正常组(P<0.05);CAEBV组B淋巴细胞、自然杀伤细胞计数低于IM组、正常组(P<0.05)。IM组与CAEBV组IgA、IgG水平高于正常组(P<0.05);但血清IgM水平三组比较差异无统计学意义(P>0.05)。结论 CD~+8T淋巴细胞计数差异是区分IM与CAEBV主要因素。检测外周血淋巴细胞亚群水平有利于临床对IM、CAEBV鉴别与诊治。
Objective To investigate the difference between peripheral blood lymphocyte subsets and immune function in children with IM and CAEBV induced by EBV infection and provide reliable data support for clinical diagnosis. Methods The clinical data of 40 pediatric patients with EBV in Zhengzhou Pediatric Hospital were retrospectively analyzed. Among them, 21 were in IM and 19 were in CAEBV. 20 healthy children were enrolled in this study. Peripheral blood and lymphocyte subsets were detected by flow cytometry. Serum immunoglobulin levels were measured by turbidimetry. Observations and comparisons were made between the three groups of blood routine and lymphocyte subsets test results, immunoglobulin test results. Results There were significant differences in blood routine test and lymphocyte subsets between the three groups (P <0.05). Among them, peripheral blood blood test results and lymphocyte subpopulation CD + 3T (P <0.05). The counts of CD ~ + 4T and CD ~ + 8T in CAEBV group were higher than that in CAEBV group and normal group (P <0.05). The counts of B lymphocytes and natural killer cells in CAEBV group were lower than those in IM group and normal group (P <0.05). The levels of IgA and IgG in IM group and CAEBV group were higher than those in normal group (P <0.05), but there was no significant difference in serum IgM level between the three groups (P> 0.05). Conclusion The difference of CD ~ + 8T lymphocyte count is the main factor to distinguish IM and CAEBV. Detection of peripheral blood lymphocyte subsets is conducive to the clinical identification of IM, CAEBV and diagnosis and treatment.