论文部分内容阅读
目的 应用四氯化碳(CCl4)制备小鼠肝纤维化模型,再给予激活素(ACT)A中和抗体,观察ACTA中和抗体对小鼠肝纤维化的抑制作用及对相关细胞因子表达的影响。方法 将健康雄性昆明种小白鼠分为5组:正常对照组、橄榄油对照组、CCl4 模型组、ACTA中和抗体组及对照抗体组。采用逆转录聚合酶链反应(RT -PCR)半定量法分析肝组织内ACTA、转化生长因子β1 (TGF -β1 )、基质金属蛋白酶组织抑制物1(TIMP -1)及血小板源性生长因子(PDGF)的表达。结果 与CCl4 模型组和对照抗体组相比, ACTA 中和抗体组肝纤维化病理学变化减轻,肝组织内ACTA、TGF -β1、TIMP -1 及PDGF的mRNA表达减少(P<0.01)。结论 ACTA中和抗体中和ACTA的作用,可能通过减轻对肝实质细胞的生长抑制和凋亡诱导而发挥抗肝纤维化作用,从而使肝纤维化形成的关键细胞—肝星状细胞的细胞外基质成分表达减少,进而使与之有关的细胞因子网络系统发生改变,肝纤维化程度减轻。
Objective To investigate the inhibitory effect of neutralizing antibody of ACTA on mouse hepatic fibrosis and the expression of related cytokines in mouse liver fibrosis model induced by carbon tetrachloride (CCl4) influences. Methods Healthy male Kunming mice were divided into five groups: normal control group, olive oil control group, CCl4 model group, ACTA neutralizing antibody group and control antibody group. The expressions of ACTA, transforming growth factor-β1 (TGF-β1), tissue inhibitor of metalloproteinase 1 (TIMP-1) and platelet-derived growth factor (TGF-β1) were detected by reverse transcription polymerase chain reaction PDGF) expression. Results Compared with CCl4 model group and control antibody group, the changes of pathological changes of liver fibrosis in ACTA neutralizing antibody group were alleviated, and the mRNA expressions of ACTA, TGF-β1, TIMP-1 and PDGF in liver tissue were decreased (P <0.01). Conclusion ACTA neutralizing antibody neutralizing ACTA may play an important role in anti-hepatic fibrosis by reducing the growth inhibition and apoptosis induction of hepatic parenchymal cells, so that the key cells of hepatic fibrosis - extracellular of hepatic stellate cells Reduce the expression of matrix components, which in turn make the relevant cytokine network system changes, reducing the degree of liver fibrosis.