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目的探讨胃电节律失常与亮啡肽的关系．方法Ｗｉｓｔａｒ大鼠６０只，随机分为模型组（ｎ＝３０）、对照组（ｎ＝２０）、药物组（ｎ＝１０）．模型组大鼠按自行设计的不规则喂养方法饲养４ｗｋ：大鼠单日正常进食，双日禁食，以打乱正常的饮食节律．浆膜下埋置银丝电极，７ｄ后同步记录胃电快波、慢波．药物组大鼠ｉｐ吗啡、纳络酮，观察对胃肌电的影响．检测对照组、实验组大鼠血浆、胃窦组织亮啡肽含量．免疫细胞化学及组织化学方法显示胃窦肌间神经丛亮啡肽免疫反应神经及胆碱能神经，并应用图象分析系统进行计量处理．结果实验组２７只出现不同类型节律失常，异常节律指数为３６６７％．ｉｐ吗啡后，大鼠均出现胃电节律失常，纳络酮可阻断此作用．模型组血浆亮啡肽含量与对照组相比无明显改变（６５３８ｎｇ／Ｌ±１９７８ｎｇ／Ｌ，５５４９ｎｇ／Ｌ±２３４１ｎｇ／Ｌ，Ｐ＞００５）；胃窦组织亮啡肽含量明显增加（５７５ｎｇ／ｇ±２１３ｎｇ／ｇ，３６２ｎｇ／ｇ±１９４ｎｇ／ｇ，Ｐ＜００１）；胃窦肌间神经丛亮啡肽免疫反应神经明显增加（３７７２７μｍ２±３０１０μｍ２，３２１６７μｍ２±２１４８μｍ２），胆碱能神经明显减少（５５４４７μｍ２±?
Objective To investigate the relationship between gastric dysrhythmia and leucovorin. Methods Sixty Wistar rats were randomly divided into model group (n = 30), control group (n = 20) and drug group (n = 10). The rats in the model group were fed 4wk according to the irregular feeding method designed by themselves: the rats were fed normally for one day and fasted for two days to disturb the normal eating rhythm. Serous buried silver electrode, 7d after synchronous recording of gastric fast wave, slow wave. Effect of ip morphine and naloxone on the gastric myoelectrical in drug group. The levels of leupeptine in the plasma and gastric antrum were detected in the control and experimental groups. Immunocytochemistry and histochemical methods showed that the leucovorin immunoreactive nerve and cholinergic nerve in the myenteric plexus of the gastric antrum were measured by image analysis system. Results There were 27 different types of arrhythmias in experimental group. The abnormal rhythm index was 3667%. After ip morphine, gastric antrum dysfunction occurred in rats, naloxone can block this effect. Compared with the control group, the content of leupeptin in the model group had no significant change (6538ng / L ± 1978ng / L, 5549ng / L ± 2341ng / L, P> 0.05) The levels of leupeptin were significantly increased (575ng / g ± 213ng / g, 362ng / g ± 194ng / g, P <001). The leucovorin immunoreactive nerve (37727μm2 ± 3010μm2,32167μm2 ± 2148μm2), cholinergic nerve decreased significantly (55447μm2 ±?