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本文旨在构建能够高效降解水稻秸秆的戴氏霉组合菌。通过兼容性试验,选取木质素降解菌灰戴氏霉H57.1菌株与纤维素降解菌合川戴氏霉H08.1菌株组合发酵降解水稻秸秆,以失重法和范氏洗涤剂法检测其对秸秆的降解效果,用胞外酶活测定法探索其产酶规律。结果表明,菌株H57.1和H08.1具有良好的兼容性;组合菌H57.1+H08.1对水稻秸秆的降解能力明显高于单一菌株,秸秆失重率高达55.7%,木质素、半纤维素和纤维素降解率分别为48.9%、72.6%和57.0%;对发酵过程产酶情况的分析进一步表明,组合菌H57.1+H08.1降解水稻秸秆的能力与其产酶能力密切相关。
The purpose of this paper is to construct a combination of molds that can efficiently degrade rice straw. Through the compatibility test, we selected lignin-degrading bacterial strain D57 and strain D58 and D80 strain to strain rice straw to degrade rice straw, Degradation effect, with extracellular enzyme activity assay to explore the law of enzyme production. The results showed that the strains H57.1 and H08.1 had good compatibility. The degrading ability of the combination strain H57.1 + H08.1 on rice straw was significantly higher than that of the single strain, and the weight loss rate of straw was as high as 55.7%. The lignin, The biodegradation rates of cellulose and cellulose were 48.9%, 72.6% and 57.0%, respectively. The analysis of enzyme production in fermentation process further indicated that the ability of the combination strain H57.1 + H08.1 to degrade rice straw was closely related to its enzyme producing ability.