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试用40 个引物对我国水稻条斑病菌、“稻短条斑病菌”和李氏禾条斑病菌等14 个代表菌株进行RAPD 分析,其中11 个引物的扩增产物表现明显的多态性, 共扩增出158 条谱带,多态性为89 .74 % 。聚类分析结果显示14 个菌株可区分为3 个类群,第1 群包括LLS2 、LLS3 、LLS4 、RS05 、R1008 、TAS和TAX;来自不同稻区水稻条斑病菌的群体结构差异明显,分属于第2 群( 如RSHai 等) 和第3 群( 如RS105 等) 。菌株DNARAPD 指纹分析和致病性测定结果证明:李氏禾条斑病菌、“稻短条斑病菌”在水稻和李氏禾上不仅可相互侵染,而且遗传背景的相似性较高,确认是同一种病菌,其与小麦黑颖病菌亲缘关系较近,但与水稻细菌性条斑病菌具有明显差异
A total of 40 primers were used to analyze 14 representative strains of pathogenic bacteria, such as S. Sclerotiorum, Scutellaria barbata and Rhizoctonia solani. RAPD analysis was carried out among 11 representative primers, of which 11 primers showed significant polymorphism 158 bands were amplified with a polymorphism of 89. 74%. Cluster analysis showed that the 14 isolates could be divided into three groups. The first group included LLS2, LLS3, LLS4, RS05, R1008, TAS and TAX. The population structure of the pathogenic bacteria, 2 groups (such as RS Hai, etc.) and the third group (such as RS105, etc.). Strain DNA RAPD fingerprinting and pathogenicity test results show that: L. spp., “K. ictalis” in rice and Lee Wo Wo not only can be mutually infected, and the genetic background of the higher similarity, It is confirmed that it is the same kind of bacteria, which has a close genetic relationship with the wheat germ, but has obvious difference with that of the rice bacterial leaf blotch